DNA Replication-Interactive Tutorial
Answer the following questions as you work through the tutorial
Introduction:
1. Describe the structure and function of DNA.
2. What is replication?
3. Who discovered the structure of DNA and in what year?
4. How does this structure of DNA allow for replication?
5. What are mutations? How are they responsible for driving evolution?
Basics:
6. Describe the difference between the two possible methods of replication. Use diagrams to help in your explanation. Which method did Watson and CrickÕs model support?
7. Explain the difference between prokaryotic and eukaryotic replication.
DNA Polymerase:
8. There are 3 DNA polymerases. List them and briefly tell what each does.
9. Why must a primer be added to a DNA strand? Use a diagram to help with your explanation.
Okazaki Fragments
10. Which direction does DNA polymerase run? Why is this a problem that needs to be overcome in replicating DNA?
11. How is this problem overcome?
The Whole Picture
12. Explain the functions of gyrase and helicase in DNA replication.
13. What role does ATP play?
14. What is the function of SSBÕs?
15. What is the function of the enzyme primase? What type of molecule is it?
16. What direction does DNA polymerase III add nucleotides?
17. In what direction is DNA copied?
18. How are the primers removed?
19. What kind of gaps are left in the strand as a result of the removal of the primers?
20. How are these gaps filled? Does this require energy?
Written by Ms. Bilic
Applewood Heights S.S.